PEI‐PLGA nanoparticles significantly enhanced the immunogenicity of IsdB_137‐361 proteins from <i>Staphylococcus aureus</i>

Introduction Staphylococcus aureus seriously threatens human and animal health. IsdB137-361 of the iron surface determinant B protein (IsdB) from S. exhibits strong immunogenicity, but its immunoprotective effect is still to be further promoted. Because PEI-PLGA nanoparticles are generated by PEI conjugate with PLGA develop great potential as a novel immune adjuvant, immunogenicity likely strengthened PEI-PLGA. Methods Here, containing proteins were prepared optimizing entrapment efficiency. Mice immunized IsdB137-361-PEI-PLGA assess their anti-S. effects. The level IFN-γ, IL-4, IL-17, IL-10 cytokines spleen lymphocytes in mice generation antibodies against serum was assessed ELISA, protective response appraised challenge. Results loaded able stimulate effectively proliferation increase secretion cytokine lymphocytes, significantly enhance serum, reduce bacterial load liver, kidney, greatly improve survival rate after Conclusion These data showed that can proteins, provide an important reference for development adjuvant.